J. Gen. Appl. Microbiol., 57, 331‒339 (2011)
نویسندگان
چکیده
Bacteria are fundamentally divided into two groups, Gram-positive and -negative, based on the chemical and physical properties of their cell walls. The classic Gram stain reaction permits the useful distinction between Gram-positive and -negative bacteria. Gram staining results largely affect the initial classifi cation of an unknown bacterium and the selection of follow-up identifi cation procedures. However, it is well known that some factors, including a faulty procedure, the age of a culture, and the composition of a cultivation medium, can infl uence the tendency of Gram-positive bacteria to decolorize, leading to misidentifi cation (Bartholomew and Mittwer, 1952; Cowan and Liston, J. Gen. Appl. Microbiol., 57, 331‒339 (2011)
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